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KMID : 0370220210650050349
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Yakhak Hoeji
2021 Volume.65 No. 5 p.349 ~ p.354
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Role of Ca2+-activated Cl? Channels in the Spiperone-mediated Melanogenesis
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Lee Yong-Soo
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Abstract
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The molecular mechanism underlying the stimulation of melanin synthesis by the antipsychotic drug spiperone(SP) was investigated in B16 melanoma cells. SP increased the melanin content and intracellular Ca2+ ion concentrationin a dose-dependent manner. Treatment with bis-(o-aminophenoxy)-ethane-N,N,N',N'-tetraacetic acid/acetoxymethyl ester, anintracellular Ca2+ chelator, significantly inhibited SP-induced melanin synthesis. SP induced considerable Cl? efflux, whichwas inhibited by niflumic acid and flufenamic acid, known Ca2+-activated Cl? channel (CaCC) blockers. Furthermore, theseCaCC blockers significantly inhibited SP-induced melanin synthesis. Thus, these results suggest that CaCCs may play animportant role in SP-induced melanin synthesis in melanoma cells.
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KEYWORD
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Spiperone, Melanin synthesis, Ca2+ signal, Ca2+-activated Cl? channel, B16 melanoma cell
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